The main components include surfactant, inorganic salt and resin.
Limitations of Procedure
The release efficiency of nucleic acid is related to whether the operator strictly follows the instructions. If not controlling the cross-contamination well in sample processing, a false positive result may be occurred. Sample testing result is related to sample collection, handling, transportation, and preservation, any mistake may lead to the false negative result.
If not controlling the cross-contamination well in sample processing, a false positive result may be occurred.
One Step To PCR
1. Preservation method
Broke the part of swab that above the collection tube of the sampled isolated virus swab, immerse the swab sample into collection tube containing sample release reagent, screw the tube cap tightly. If the nucleic acid cannot be extracted in time, the sample can be stored at 15-25℃ for I days, 2-8℃ for 3 days, -20±5℃ for 2 weeks or stored at-80±10℃ for long-term storage.
2. Nucleic acid release
1) Place the collection tube on the vortex oscillator and shake vigorously for 15 seconds.
2) After completing the previous step, let it stand at room temperature for 15 minutes, and then take the supernatant for PCR detection.